Rat Interleukin 10, IL-10 ELISA Kit

Full product name

Interleukin 10

Code

E0108Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.51pg/ml

Detection range

3-900pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

UniProt accession

P29456

MASS?Da?

20426

GeneID

25325

Synonyms

CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

Gene names

IL10

Research area

Immunology; Cancer

Target protein

IL10