Rat Tumor Necrosis Factor ?, TNF-A ELISA Kit

Full product name

Tumor Necrosis Factor alpha

Code

E0764Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.51ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

UniProt accession

P16599

MASS?Da?

25,806

GeneID

10369438024835

Synonyms

Cachectin;C-domain 1;C-domain 2;ICD1;ICD2;Intracellular domain 1;Intracellular domain 2;N-terminal fragment;NTF;TNF A;TNF alpha;TNFA;TNF-a;TNF-alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor necrosis factor, membrane form;Tumor necrosis factor, soluble form

Gene names

TNFA

Research area

Immunology; Signal Transduction; Cancer; Microbiology

Target protein

TNFA